Debasish B Krishnatreya

3 Questions 8 Answers 0 Followers

Questions related from Debasish B Krishnatreya

How to construct primer for U6snRNA reverse transcription?

I am currently working on tea miRNAs. For RT-qPCR analysis i need to use the U6 as control. I have worked out the primers for miRNAs, and also have forward and reverse primer for all. But how can...

09 September 2019 8,243 4 View

How to filter out reads which have less than 100 amino acid long ORFs?

I am working on a transcriptome file and now i need to retrieve only the reads that have ORFs shorter than 100 amino acids and discard the reads which have viable ORFs (more than 100 amino acids)....

05 May 2019 8,948 2 View

How to find out the non-aligned reads of a query fasta file consiting of sRNA sequences after running standalone blast against miRBase?

I am working with miRNAs in tea, and for finding out conserved and novel miRNAs, I have blasted the filtered data set of sRNA sequences against the miRBase. But the output only shows the aligned...

11 November 2018 7,754 5 View