Debasish B Krishnatreya

3 Questions 7 Answers 0 Followers

Questions related from Debasish B Krishnatreya

What does the part "mature miRBase miRNAs not detected by miRDeep2" actually mean in a miRDeep2 analysis? Should those hits be included in results?

After completion of a miRDeep2 analysis, the results are generated in three different parts, viz., 1) novel miRNAs predicted by miRDeep2, 2) mature miRBase miRNAs detected by miRDeep2 3) mature...

01 July 2022 5,854 0 View

How to construct primer for U6snRNA reverse transcription?

I am currently working on tea miRNAs. For RT-qPCR analysis i need to use the U6 as control. I have worked out the primers for miRNAs, and also have forward and reverse primer for all. But how can...

10 September 2019 8,359 3 View

How to filter out reads which have less than 100 amino acid long ORFs?

I am working on a transcriptome file and now i need to retrieve only the reads that have ORFs shorter than 100 amino acids and discard the reads which have viable ORFs (more than 100 amino acids)....

29 May 2019 4,132 2 View