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I am currently working on tea miRNAs. For RT-qPCR analysis i need to use the U6 as control. I have worked out the primers for miRNAs, and also have forward and reverse primer for all. But how can...
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I am working on a transcriptome file and now i need to retrieve only the reads that have ORFs shorter than 100 amino acids and discard the reads which have viable ORFs (more than 100 amino acids)....
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