7 Questions 16 Answers 0 Followers
Questions related from Debasis Sahu
I have designed a few pairs of sgRNA oligos using both CHOPCHOP and CRISPOR. I checked for their alignments within themselves. However, when I did the Primer-BLAST using both oligos (excluding the...
28 June 2021 673 5 View
I am actively engaged in the research work pertaining to phytochemicals and finding out the mechanism of their action. The diseases in consideration are autoimmune and inflammatory in nature. I...
10 May 2018 2,775 4 View
I guess that the reducing sample buffer might have expired so that the b-mercaptoethanol is no longer stable which in turn did not result in reduction and the proteins could not migrate properly...
22 May 2017 1,271 5 View
After the identification of numerous proteins to be deregulated in a diseased tissue using iTRAQ what should be the criteria to select a few proteins for their validation using Western...
10 July 2016 2,050 3 View
I am planning to screen an array of potential anti-inflammatory and anti-oxidant agents in macrophage cell lines such as RAW 264.7 or J774. The cells will be stimulated with LPS to look for...
01 September 2014 6,649 19 View
I have got a reviewer’s comment on my statistical analysis. My present analysis is based on t-test and the reviewer is asking for reanalysis using non-parametric test. Please suggest the...
30 March 2014 5,403 14 View
.
08 February 2014 3,504 23 View