Debasis Sahu

6 Questions 12 Answers 0 Followers

Questions related from Debasis Sahu

Is it necessary to do primer-BLAST for sgRNA oligos designed for CRISPR knockout experiment?

I have designed a few pairs of sgRNA oligos using both CHOPCHOP and CRISPOR. I checked for their alignments within themselves. However, when I did the Primer-BLAST using both oligos (excluding the...

28 June 2021 625 5 View

In one of my Western blot results all my protein bands (33- 57 kDa) appeared at the top (protein marker was fine) 175 to 270 kDa. Reason?

I guess that the reducing sample buffer might have expired so that the b-mercaptoethanol is no longer stable which in turn did not result in reduction and the proteins could not migrate properly...

22 May 2017 1,221 5 View

What are the criteria of selection of proteins for western blot validation after global proteome analysis?

After the identification of numerous proteins to be deregulated in a diseased tissue using iTRAQ what should be the criteria to select a few proteins for their validation using Western...

10 July 2016 2,001 3 View

What are the methods to stimulate cells for inducing inflammatory mediators, apart from LPS?

I am planning to screen an array of potential anti-inflammatory and anti-oxidant agents in macrophage cell lines such as RAW 264.7 or J774. The cells will be stimulated with LPS to look for...

01 September 2014 6,619 19 View

Which particular non-parametric test should be carried out in case of multiple group comparisons? Which software should be used for this?

I have got a reviewer’s comment on my statistical analysis. My present analysis is based on t-test and the reviewer is asking for reanalysis using non-parametric test. Please suggest the...

30 March 2014 5,375 14 View

Are autoantibodies produced more often by alteration in post-translational modifications of proteins than any other method?

08 February 2014 3,481 23 View