Debasis Sahu

3 Questions 4 Answers 0 Followers

Questions related from Debasis Sahu

Is it necessary to do primer-BLAST for sgRNA oligos designed for CRISPR knockout experiment?

I have designed a few pairs of sgRNA oligos using both CHOPCHOP and CRISPOR. I checked for their alignments within themselves. However, when I did the Primer-BLAST using both oligos (excluding the...

28 June 2021 613 5 View

In one of my Western blot results all my protein bands (33- 57 kDa) appeared at the top (protein marker was fine) 175 to 270 kDa. Reason?

I guess that the reducing sample buffer might have expired so that the b-mercaptoethanol is no longer stable which in turn did not result in reduction and the proteins could not migrate properly...

22 May 2017 1,204 5 View

What are the criteria of selection of proteins for western blot validation after global proteome analysis?

After the identification of numerous proteins to be deregulated in a diseased tissue using iTRAQ what should be the criteria to select a few proteins for their validation using Western...

10 July 2016 1,985 3 View