Anyone knows about the dose of puromycin for selecting lentiviral vector transduced medullo cells (D556 or UW288-1)?

Anybody done puromycin selection on medullo cells (D556 or UW288-1) transduced with lentiviral vector for expression of a gene? What is the ideal dose of puromycin for selecting medullo cells

07 July 2016 6,940 4 View