Debajyoti Saha

3 Questions 1 Answers 0 Followers

Questions related from Debajyoti Saha

Issues with pET22b vector, What should I do?

I have double digested and gel-purified pET22b vector and put ligation with my insert in 1:3 & 1:5 and kept at room temperature and 16C overnight with both, transformed into DH5a, plated them into...

24 December 2024 6,911 3 View

How can I measure protein concentration when my buffer (media) is colored?

I am expressing a extracellular protein and the media is colored. So when I used nanospectrophotometer, for blank (media) it showed high absorbance >340 nm and couldnot measure it. So I diluted...

17 November 2023 2,024 0 View

Can I digest a sequence having one restriction site for SacII?

Dear All, I want to linearize a plasmid by SacII, which have one restriction site for SacII. Should I keep it for 3-4hr at 37°C for getting at least 90% efficiency? Suggest me any better...

21 September 2023 3,979 2 View