Deanna M Santer

5 Questions 9 Answers 0 Followers

Questions related from Deanna M Santer

Is there a way to calculate ddCts/fold changes if your gene of interest is undetectable in unstimulated but then detectable after stimulation?

Hi all This must have happened to others before. For example an IFN stimulated gene like MX1 goes from undetectable (>40 cycles) but then after IFN alpha stimulation the Ct is now 28. Both...

03 March 2018 7,147 5 View

What is the best way to measure virus specific IgG from in vitro human cell stimulations?

We are setting up a variety of virus stimulations as positive controls for adding to human PBMCs. My goal is to measure IgG induced by the virus so while total IgG is easy to measure via ELISA,...

06 June 2014 8,903 5 View

Do you find isotype control background goes up with stimulation?

I've seen this for awhile and just assume it's normal as I add in Fc block to prevent extra background but thought I would see if others also see what I am seeing. Maybe others dont use isotypes...

04 April 2014 3,981 3 View

Is there any way to remove heparin from PBMC DNA so it can be used for qPCR genotyping?

We already purified DNA from human PBMCs that were originally collected in sodium heparin tubes. Since we know heparin can affect qPCR reactions (which we would like to use DNA for SNP analysis),...

04 April 2014 700 23 View

Has anyone been successful using filter plates to surface stain/wash human PBMCs for flow cytometry?

I am curious if anyone has been successful using a vacuum manifold to wash FACS samples instead of centrifugation to save lots of time if stain samples in a 96 well millipore filter plate? We...

03 March 2014 1,196 6 View