Dawid Zyla

4 Questions 22 Answers 0 Followers

Questions related from Dawid Zyla

What are the best 3 different reporter proteins as fusion partners for structural studies?

Hello, I am looking for 3 different reporter proteins (fluorescence / chemiluminesence / enzymes) which could have a fused tag on C-terminus (N -reporter protein - tag - C). I want to use them for...

07 July 2016 4,272 2 View

Can someone provide me with some advice on using BS3 crosslinker and DTT buffer?

I would like to perform a crosslinking experiment using BS3 crosslinker. My protein has 3 cysteines, but no S-S bridge, so I have to keep my protein in buffer with 5mM DTT. Can I perform the...

07 July 2013 5,082 10 View

Modelling of unstuctured proteins

I would like to ask if anyone knows good and possibly fast programs for modelling of unstructured proteins like transcription factors. I need to check if my protein could interact with other...

12 December 2012 3,110 22 View

Reason for protein precipitating in buffer during dialysis?

I have quite an annoying problem - I'm purifying a protein with a His-tag with elution buffer (6M urea, DTT, 300mM imidazole). In elution buffer, the protein is in the supernatant and everything...

08 August 2012 1,928 14 View