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Questions related from Dawid Deneka
I have heard some rumors that the HRV-3C protease can recognize and cleave ENLYFQG peptide, which is a consensus sequence cleaved by the TEV protease. Can anyone confirm or suggest some literature...
24 July 2018 1,523 4 View
I wanted to analyze the phosphorylation of a certain mammalian protein. It is a standard procedure to add phosphatase inhibitors to extraction buffers to block the process of dephopshorylation....
13 March 2018 9,136 4 View
Dear all, I am overexpressing my protein of interest in HEK293T. The protein is supposed to be soluble with no particular localization signal, which means it is expected to be in the cytoplasm....
25 June 2017 6,128 3 View
Dear all, I would like to ask what is the maximal achievable resolution of ion exchange chromatography in the matter of pI values. In particular I am wondering whether I will be able to separate...
12 October 2016 5,781 3 View
Dear Scientist, My actual task is to incorporate some membranes proteins (multipass, 7TM, singlepass) into liposomes and nanodiscs. Native membranes are polarized structures with different lipid...
01 August 2014 3,463 8 View
I am planning to prepare a 100-1500bp DNA marker using endonuclease digestion of a special plasmid. I am wondering if the presence of sticky ends can affect gel migration of generated fragments....
08 October 2013 877 12 View
I would like to clone a 7bkp insert into a pUC18 vector (2,7kbp). Do you know if it may be somehow problematic? I mean ligation or transformation efficiency, insert stability or any other...
27 March 2013 3,064 2 View
I am interested in preparing a phagemid library. I would like to know if it is very expensive (more or less) and if it needs lots of lab experience. I would be grateful for any answer!
07 October 2012 5,092 6 View
