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Questions related from Dawid Deneka
I have heard some rumors that the HRV-3C protease can recognize and cleave ENLYFQG peptide, which is a consensus sequence cleaved by the TEV protease. Can anyone confirm or suggest some literature...
24 July 2018 1,443 4 View
I wanted to analyze the phosphorylation of a certain mammalian protein. It is a standard procedure to add phosphatase inhibitors to extraction buffers to block the process of dephopshorylation....
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Dear all, I would like to ask what is the maximal achievable resolution of ion exchange chromatography in the matter of pI values. In particular I am wondering whether I will be able to separate...
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Dear Scientist, My actual task is to incorporate some membranes proteins (multipass, 7TM, singlepass) into liposomes and nanodiscs. Native membranes are polarized structures with different lipid...
01 August 2014 3,422 8 View
I am planning to prepare a 100-1500bp DNA marker using endonuclease digestion of a special plasmid. I am wondering if the presence of sticky ends can affect gel migration of generated fragments....
08 October 2013 818 12 View
I would like to clone a 7bkp insert into a pUC18 vector (2,7kbp). Do you know if it may be somehow problematic? I mean ligation or transformation efficiency, insert stability or any other...
27 March 2013 3,012 2 View
I am interested in preparing a phagemid library. I would like to know if it is very expensive (more or less) and if it needs lots of lab experience. I would be grateful for any answer!
07 October 2012 5,043 6 View
