6 Questions 21 Answers 0 Followers
Questions related from Dawid Deneka
I have heard some rumors that the HRV-3C protease can recognize and cleave ENLYFQG peptide, which is a consensus sequence cleaved by the TEV protease. Can anyone confirm or suggest some literature...
24 July 2018 1,425 4 View
I wanted to analyze the phosphorylation of a certain mammalian protein. It is a standard procedure to add phosphatase inhibitors to extraction buffers to block the process of dephopshorylation....
13 March 2018 9,060 4 View
Dear all, I would like to ask what is the maximal achievable resolution of ion exchange chromatography in the matter of pI values. In particular I am wondering whether I will be able to separate...
12 October 2016 5,694 3 View
I am planning to prepare a 100-1500bp DNA marker using endonuclease digestion of a special plasmid. I am wondering if the presence of sticky ends can affect gel migration of generated fragments....
08 October 2013 811 12 View
I would like to clone a 7bkp insert into a pUC18 vector (2,7kbp). Do you know if it may be somehow problematic? I mean ligation or transformation efficiency, insert stability or any other...
27 March 2013 3,002 2 View
I am interested in preparing a phagemid library. I would like to know if it is very expensive (more or less) and if it needs lots of lab experience. I would be grateful for any answer!
07 October 2012 5,034 6 View
