David William Waters

4 Questions 5 Answers 0 Followers

Questions related from David William Waters

What is a suitable explanation for increased ATP production if there is no indication of increased mitochondrial mass?

I have evidence of mitochondrial dysfunction (Seahorse assay) but no evidence for an increase in mitochondrial mass (fluorescence assay). What might be causing the dysfunction, particularly...

03 March 2018 6,821 5 View

Protocol for assessing mitochondrial mass in fibroblasts using MitoTracker Green

Hi, I’m after a protocol for assessing mitochondrial mass in fibroblasts using MitoTracker Green and Flow. Can anyone help please? I have seen a couple of protocols online, for different cell...

07 July 2017 170 1 View

Has anybody used a B-galactosidase antibody for WB with lysates from human cells?

I want to identify senescent cells in culture and have been using the B-Gal staining kit (Cell Signalling) with inconclusive results. I would like to confirm my observations using another...

01 January 2017 3,537 3 View

How to quantify SA-B-Gal staining?

I’m using the Senescence β-Galactosidase Staining Kit from Cell Signalling to identify senescent cells. Does anyone know a method of reliably quantifying the stained cells in culture? I perform...

10 October 2016 8,757 3 View