David Shire

4 Questions 4 Answers 0 Followers

Questions related from David Shire

Can I reconstitute dried peptides without trifluoroacetic acid or formic acid?

I want to quantify the recovery of tryptic peptides from a desalting method that I intend to use for preparing samples for LC-MS. My tryptic digests started out in ammonium bicarbonate and were...

08 August 2019 8,348 3 View

How can I prevent proteins from precipitating in anion exchange column?

Hi all, I'm trying to do a gradient elution of whole-cell extracts from marine phytoplankton with anion exchange chromatography to isolate a protein of interest. My one specification is that the...

03 March 2017 1,009 6 View

Why do I have floating cell clumps after centrifugation?

We've been trying to spin down a marine microalgae, Phaeocystis globosa. Other studies report centrifuging dense cultures at 100 x g for 10 min. We've been trying all sorts of speeds, durations,...

02 February 2016 2,753 5 View

Can a metal cofactor be cross-linked into a protein?

Suppose I have, say, a zinc-structural metalloprotein. Can I ensure that the metal and protein will stay associated under not super gentle conditions (during electrophoresis, for example) by...

01 January 2016 9,381 5 View