David Shields

4 Questions 7 Answers 0 Followers

Questions related from David Shields

Should I culture PBMCs overnight between thawing them from frozen, and separation on nylon wool?

Hi guys! I'm planning on performing a crude isolation of B cells from PBMCs by nylon wool before I stimulate them with CD40L, IL-4 and IL-10 (to induce production of all Ig subtypes). The majority...

06 June 2015 2,440 7 View

How long does BigDye terminator premix last at RT in light?

Hi guys! I'm having to do a LOT of DNA sequencing and it appears the fastest way for me to do that is to set up 96 sequencing reactions and run them through the thermocycler, then taking them out...

08 August 2014 233 5 View

How long can lithium heparinized blood be stored before PBMC harvesting and flow cytometry?

Hi guys, So I'm receiving blood samples each day so that I can harvest PBMCs for flow cytometric analysis (and neutrophils for possible DNA extraction). The diagnosticians that take the blood for...

08 August 2014 3,705 7 View

Does anyone have experience with batch sequence alignment programs?

I'm trying to find a program that will do batch sequence alignment. Basically what I'm doing is, I have a reference sequence obtained from NCBI and I have sequencing files (.ab1) from DNA samples...

06 June 2014 6,086 6 View