David Sassoon

2 Questions 15 Answers 0 Followers

Questions related from David Sassoon

What is the best system (and company if possible) for viral Cre delivery to primary (proliferative) cells in culture?

We have the conditional allele mouse that has been tested with several Cre and CreER mice and it works. However, we can move faster if we just infect Cre directly into the cells but we need high...

05 May 2015 8,127 3 View

If siRNA and/or shRNA knock down gene expression are at undetectable levels, are these results less significant than using a conditional KO mouse?

This question was asked to me by an editor of a journal. I think both are valid models but the editor feels that KO mice are better.

12 December 2012 6,965 18 View