David Kainer

3 Questions 7 Answers 0 Followers

Questions related from David Kainer

Why does the barcode appear at the start of both reads in pair?

Hi helpful people, I have paired-end sequence data for pooled samples which were library prepped with a custom inline barcoded P5 adapter. So the first 6 bases of the R1 read should contain the...

09 September 2015 7,982 2 View

Best way to blast a few thousand short fasta sequences against millions of fastq short reads?

Hi All, I have: A) a fasta file containing a few thousand short sequences (40bp each) B) an 8Gb fastq.gz file containing millions of unassembled illumina paired end reads (100bp each) I would like...

02 February 2015 3,629 9 View

What is a good tool for phasing genotypes in a VCF file?

I have a VCF file with unphased genotypes. I would like to analyze LD, which I understand is best done with phased genotypes, and hence phased haplotypes. I would like to generate a new VCF file...

05 May 2014 1,000 11 View