David Hughes

2 Questions 4 Answers 0 Followers

Questions related from David Hughes

Would someone be able to provide assistance with alizarin red s/mineralization assay with SaOS-2 cells?

I'm currently trying to induce and identify mineralization in my SaOS-2 cells. The issue I'm having is that the whole well will stain red, and of course this intensity depends on how long the...

01 January 2020 6,710 4 View

Would someone be able to help with optimising a Western Blot for Collagen I from whole cell lysates of Saos-2 osteoblast cells?

I'm looking to run a Western Blot to identify expression of collagen type I from whole cell lysates of Saos-2 osteoblast cell lines. I've listed reagents at the bottom of the post. Experimental...

09 September 2019 2,371 6 View