Extrusion of a POPC lipid dispersion to get 800-1000 A vesicles and getting lots of 'Polys' (vesicle in vesicle). How do I reduce Poly's in the prep?

I am making preformed lipid vesicles for use in protein reconstitution and after 5-7 x freeze-thaw cycles with N2l to ~ 10-15C, extrusion through a 0.2 um filter x 10, and extrusion through 2 x 80...

10 October 2019 1,184 3 View