RNA precipitation yields a pellet. Nanodrop thinks 5ug/uL RNA, but gel shows very dim band?

I am doing a Megascript RNA precipitation reaction using Sp6 polymerase.  After the reaction, I add 1uL DNase for 15mins, then 1uL EDTA to quench. After adjusting volume to 50uL, I add 5uL 5M...

12 December 2015 1,487 2 View