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Questions related from David William Waters
Hello, I'd like to FACS sort antigen specific B cells from spleens of OVA sensitised mice. Can anybody point me in the right direction for a protocol for this please? Any ideas on how many...
28 August 2019 7,007 2 View
Hi, I'm culturing mouse B cells, stimulated, but am having difficulty counting them due to the aggregates/ foci they are forming in culture. These aggregates are difficult to disassociate for...
26 July 2019 1,945 0 View
I have evidence of mitochondrial dysfunction (Seahorse assay) but no evidence for an increase in mitochondrial mass (fluorescence assay). What might be causing the dysfunction, particularly...
13 March 2018 4,551 4 View
Hi, I found a post on RG from 2014 regarding this system but I wondered if there had been any improvements in the technology since. The general consensus in 2014 was that the system was not fit...
09 February 2017 4,233 1 View
I want to identify senescent cells in culture and have been using the B-Gal staining kit (Cell Signalling) with inconclusive results. I would like to confirm my observations using another...
10 January 2017 3,534 3 View