David William Waters

5 Questions 4 Answers 0 Followers

Questions related from David William Waters

Advice on an OVA sensitisation protocol to isolate mouse antigen specific B cells?

Hello, I'd like to FACS sort antigen specific B cells from spleens of OVA sensitised mice. Can anybody point me in the right direction for a protocol for this please? Any ideas on how many...

28 August 2019 7,007 2 View

Culturing mouse B cells, counting and seeding density advice please?

Hi, I'm culturing mouse B cells, stimulated, but am having difficulty counting them due to the aggregates/ foci they are forming in culture. These aggregates are difficult to disassociate for...

26 July 2019 1,945 0 View

What is a suitable explanation for increased ATP production if there is no indication of increased mitochondrial mass?

I have evidence of mitochondrial dysfunction (Seahorse assay) but no evidence for an increase in mitochondrial mass (fluorescence assay). What might be causing the dysfunction, particularly...

13 March 2018 4,551 4 View

Update on the ProteinSimple Wes capillary western blot system?

Hi, I found a post on RG from 2014 regarding this system but I wondered if there had been any improvements in the technology since. The general consensus in 2014 was that the system was not fit...

09 February 2017 4,233 1 View

Has anybody used a B-galactosidase antibody for WB with lysates from human cells?

I want to identify senescent cells in culture and have been using the B-Gal staining kit (Cell Signalling) with inconclusive results. I would like to confirm my observations using another...

10 January 2017 3,534 3 View