David Ruiz Pardo

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Questions related from David Ruiz Pardo

How do polyacrylamide gradient gels work in SDS - PAGE?

In SDS - PAGE is common to have a stacking and a resolving gel, each with different pH. Is there such thing in a gradient polyacrylamide gel? Since it is only one gel... Is there a stacking...

06 June 2023 4,424 6 View

Why do different buffers produce different resolutions of the same protein in gel electrophoresis?

For example why Tricine - Tris gives better resolution than Glycine - Tris in this papper? https://www.nature.com/articles/nprot.2006.4 I know it is related to the ion fronts but I don't truly...

19 May 2023 1,268 3 View