David Peeney

2 Questions 3 Answers 0 Followers

Questions related from David Peeney

Looking for advice with regards to elution from neutravidin-agarose?

I am having an issue with elution from neutravidin-agarose. I know my sample contains biotinylated proteins, since running an immunoblot with neutravidin-HRP shows excess signal. However, after...

23 March 2020 9,298 2 View

Is there a cell/tissue structure stain that is compatible with immunofluorescence?

Hi all... I am fixing and sectioning spheroids for immunofluorscence. The biggest problem we have is finding the spheroids in the paraffin blocks. There are some pretty nice protocols for IHC,...

11 July 2019 2,678 1 View