David Otaegui

5 Questions 4 Answers 0 Followers

Questions related from David Otaegui

Which is the best tool for analyze DNA-seq data From ITS region (Where Can We found a good library fro the aligment)?

I am trying to find a bioinformatic tool to analyze FASTAq coming from ITS region

11 January 2019 823 5 View

Have anyone experience about the effect of time in the expression of CircRNA?

We have some experience in different splicing in some RNA depending of the time that the blood is in the bench waiting for the RNA extraction. We are wondering if that can occurs also with circRNA.

16 May 2018 5,595 3 View

Does anyone has an idea about the coverage needed to study CircRNA in RNA( enriched in circRNA by rRNA depletion and RNASe R enrichement) from PBMC?

How coverage will I need in a NGS study to cover all circRNA? The sample will be RNA from PBMC, previously treated with rRNA depletion and RNAse R (to enrich the sample in circ)

21 April 2016 2,012 2 View

Digital PCR, Qx200 vs Quantstudio 3D; Which is the best?

Digital PCR is coming to the labs to stay and the transference to clinical applications will be really fast. In this scenario choosing the right instrument will be critical. It seems that the...

22 May 2014 8,468 13 View

We have some troubles with the identification of C9orf72 expansion by PCR. Has anyone a better way to detect it?

C9orf72 is an expansion related to some neurological diseases such as ALS or dementia.

18 January 2013 5,502 2 View