David J Martino

4 Questions 8 Answers 0 Followers

Questions related from David J Martino

Can you store cells in RNAlater for ATAC-seq?

Dear crowd, Can haematopoetic cells sorted by flow cytometry safely be stored in RNAlater for ATAC seq? Any risk of compromising the integrity of nuclei? Thanks

05 April 2018 1,731 3 View

DMSO equilibration time for PBMC - what are the risks and benefits?

The Nalgene/Nunc cyropreservation manual suggests equilibrating animal cells in cryoprotectant (DMSO) for 15 - 45min at ambient temperature to allow the cryoprotectant to penetrate the cells...

12 August 2016 3,657 2 View

What is the best software for managing biorepositories?

We're looking for alternatives to FreezerPro software for management of biospecimens. Does anyone have a particular platform they find useful, and if so why? Thanks

06 April 2016 8,733 1 View

Can we preserve labelled cells for flow cytometry long term?

How long can live cells labelled with fluorescent antibodies and fixed for subsequent analysis last before the fluorochromes degrade? Are there any methods to label cells for cell...

24 August 2015 2,857 11 View