David E. Lee

3 Questions 4 Answers 0 Followers

Questions related from David E. Lee

Can anyone help with fluorescent tagged miRNA inhibitor plasmid?

I am currently doing cell experiments with GFP linked miR plasmids to overexpress microRNAs. I have done reciprocal experiments using anti-miR microRNA inhibitors from life technologies but I am...

05 April 2015 635 2 View

Does Hoechst fluorescent counterstain of DNA in live adherent cells allow the cells to continue growing normally? Is there a better alternative?

I would like to do a time course experiment in C2C12 mouse myoblast cells over a ~7 day period. They will be transfected with GFP and I would like a nuclear fluorescent stain such as DAPI or...

03 November 2014 2,986 5 View

Any idea as to why I might have some highly variant 18S Ct values while other targets amplify normally?

Troubleshooting issues: In RT-PCR, we have had trouble with our usual amplifications of 18S using SYBR green primers. We have tried multiple, already tested and confirmed sets of primers, and...

04 May 2014 3,959 4 View