David E. Lee

2 Questions 4 Answers 0 Followers

Questions related from David E. Lee

Can anyone help with fluorescent tagged miRNA inhibitor plasmid?

I am currently doing cell experiments with GFP linked miR plasmids to overexpress microRNAs. I have done reciprocal experiments using anti-miR microRNA inhibitors from life technologies but I am...

05 April 2015 601 2 View

Any idea as to why I might have some highly variant 18S Ct values while other targets amplify normally?

Troubleshooting issues: In RT-PCR, we have had trouble with our usual amplifications of 18S using SYBR green primers. We have tried multiple, already tested and confirmed sets of primers, and...

04 May 2014 3,923 4 View