David Hunt

2 Questions 2 Answers 0 Followers

Questions related from David Hunt

How to utilise PCR in order to add C-terminal 6xhis tags to genes of interest?

Hi all, I have recently been attempting to add a C-terminal 6xhis tag to my gene of interest. My reverse primer for this consisted of ~20bp homology to the end of the ORF, omitting the STOP codon...

16 January 2024 562 5 View

Why does my prestained protein ladder stop running straight toward the bottom of my gel?

Hi all, Just wondering if anyone has experienced similiar issues when running SDS-PAGE gels? The ladder stops running straight toward to bottom of the gel? Ponceau staining of the nitrocellulose...

19 May 2023 9,084 4 View