David Gorrie

6 Questions 3 Answers 0 Followers

Questions related from David Gorrie

What is wrong with Site-Directed Mutagenesis?

I am following the NEB protocol to perform SDM with Q5 and NEB HiFi Assembly. I am making a two base pair change. First picture is my Primers designed with NEBaseChanger. Second Picture is my...

25 June 2024 5,953 4 View

AutoDock4 Docking with Flexible Residues, Fatal Error?

Hello, I am using Autodock4 and AGFR to perform in silico docking experiments with a small molecule, triptolide, and a human protein, XPB (ERCC3). I am running on windows 11. I only experience...

12 January 2024 3,178 3 View

AutoDock Vina Covalent Docking, is pybel.py broken?

Running Linux ubuntu in windows 11 using WSL I have anaconda3 installed I'm running the covalent docking tutorial for autodock vina available...

22 December 2023 6,507 1 View

Off Target Thrombin Cleavage?

Hello All, I am attempting to cleave a purified fusion protein with thrombin. I have assembled the following reaction: 11.5ul Thrombin (550 US Units/mL), 13.2ul fusion protein (19mg/mL), 100ul...

19 February 2022 8,071 0 View

Can I culture cells with PI in the media?

I want to culture cells after staining with propidium iodide, so there will be PI in the media in which the cells are cultured. Can I do this or will the PI kill the cells? basically I want to...

23 July 2021 4,226 1 View

Can someone tell me why my plasmids are running at different sizes on the gel?

Hi all, I streaked out a glycerol stock, let it grow overnight. Then i picked single colonies and inoculated 2 cultures in LB 500mL each with a single colony using sterile toothpick. I grew them...

06 November 2020 5,031 4 View