Does anyone have successful experience with immunofluorescence for PSD proteins (NMDAR subunits, PSD-95…) on brain cryosections (not in cultures)?.

I tried it with long incubation times (3 days for primary antibodies), 0,3% Triton, heat-, enzymatic- or citrate buffer-induced antigen retrieval but it doesn't work. Any advice about the protocol?

07 December 2012 9,745 19 View