David Dainko

4 Questions 1 Answers 0 Followers

Questions related from David Dainko

Issues with liquid exchange in nucleosome dialysis?

In order to reconstitute nucleosomes, I mixed DNA with histone octomers and set them up for a 16-hour dialysis to go from a NaCl concentration of 2 M to 50 mM. I used SnakeSkin Dialysis Tubing at...

30 April 2025 8,778 0 View

No Results for Bottom-Up digestion of FFPE proteins?

I had an issue with the results concerning a bottom-up experiment using tissues preserved in FFPE. The FFPE samples (1 kidney and 4 thymus tissues) were de-paraffinized by placing them in...

06 November 2023 6,958 1 View

Are these valid results from stable isotope feeding of a fungal NRPS product?

We are attempting to validate the predicted biosynthesis of a fungal non-ribosomal peptide synthase (NRPS) product via mass spec. The H+ ionized product produces a m/z at 433.2443. Looking at the...

14 November 2022 7,519 0 View

Smearing and Poor Resolution on Agarose Gel?

The quality of my gel has been progressively getting worse. I am only attempting to check the quality of the DNA, so even in an example such as this, I can still confirm the topmost band that I...

21 February 2022 9,349 1 View