David Hughes Burk

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Questions related from David Hughes Burk

Why do we see uneven picrosirius red (PSR) staining across tissue sections?

FFPE tissue sections of various murine tissues including the heart and liver, when stained using Polysciences' PSR kit (Cat# 24901), display 'blotches' or regions where there is a clear difference...

08 December 2022 7,379 3 View

Has anyone encountered an issue with Brilliant Violet 421 (BV421) not being retained or diffusing after mounting in Prolong Diamond or Vectashield?

We've been working to incorporate BV421 and BV480 into our fluorescence imaging workflow. While both secondary antibodies (Jackson Immunoresearch) work well at labeling targeted antigens, we've...

03 August 2021 1,099 1 View

How to avoid bright non-specific labeling when doing iDISCO+ staining and light sheet imaging?

We routinely use the iDISCO+ and Adipoclear techniques followed by imaging on a light sheet system and I find very bright punctate labeling around the outside of tissues such as brain or fat as...

09 December 2020 1,764 1 View