Darshan Panchariya

2 Questions 8 Answers 0 Followers

Questions related from Darshan Panchariya

How do I make a Taqman Gene expression assay mixture?

Hi everyone, I am tryingt to use Taqman probes for qPCR of isoforms. The Taqman assay guide on the website mentions using an alliquot of a 20X reaction made from a stock of 60X or 40X. However, I...

15 July 2025 4,930 3 View

Are the sitting sequences added before the PCR primers arbitrary or specific to restriction endonucleases?

As I have started to learn primer designing, I have come across terms like restriction sites, sitting sequences, etc. I wanted to know if sitting sequences added before the restriction sites are...

26 November 2021 1,659 3 View