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I've been testing the expression of certain protein N-term tagged GST/Ct-term Histag in E.coli BL21 DE3 pLysS that is quite susceptible to proteolytic cleavage so has to be extracted after 3 hours...
08 July 2017 517 3 View
I've been trying to express eukaryotic phosphatase in E.coli bl21 de3 Plyss. I had initial success (30 mg) but now it seems that all I get is lac repressor when purifying the next batches. Growth...
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