Dargham Hammad

4 Questions 3 Answers 0 Followers

Questions related from Dargham Hammad

What the reasons for smears in PCR negative control?

I have been getting smears in my negative control and clear bands in the positive control. What could be the cause?

04 April 2017 394 0 View

I am working with 16s rRNA , my amplicon size is 390 bp with 315f-806r , however I have got 350 bp , is it normal?

I am working with 16s rRNA , my amplicon size is 390 bp with 315f-806r , however I have got 350 bp , is it normal? please , if any one know answer , replay to me

12 December 2016 9,616 3 View

What is disadvantages of barcoded primers?

I used barcoded primers to amplify 16s rRNA, however, no amplify in my gel with amplification just for positive control, I hope to know main reason for this.best wishes

10 October 2016 4,819 0 View

What is the problem in line 17 of my gel?

I have problem with my gel i hope to find someone to help me, there is smeared band in with amplification of S16 rNA in whole blood samples. i have put gel picture in attached file , the problem...

03 March 2016 8,931 5 View