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Questions related from Darellynn Oo
Hi everyone, I made a mistake when I computed how much antibody I needed for my FACs, so I have an excess of exactly 10 reactions. Since they are so expensive and our lab is so poor, I was...
28 October 2020 3,780 3 View
Hello fellow researchers, I am having trouble with H&E staining. My slides end up blurry and unable to focus. I am following what I was taught, samples fixed in 4% PFA overnight, sucrose...
04 June 2019 619 5 View
I am developing a FACs B cell panel for my experiments and will need about 7-8 colours. We use C57BL/6 mice. I am trying to match my fluorochrome brightness and size to the abundance of markers on...
20 March 2019 714 1 View
Dear All, I am taking over the Master's student's project and I am a new PhD student. We have experiments involving tumour implantation in mice. The MSc student is used to scraping cells down...
18 March 2019 5,489 3 View
Hi, I used to work with T cells which I was told are much more resistant to cryopreservation. B cells on the other hand are apparently more sensitive and the viability after thawing is poor,...
04 July 2018 4,367 3 View
Dear Researchers, I need a comprehensive guideline to follow to determine analysis cut-off points (e.g.: number of nuclei per sample, percentages to consider a deletion present, false +/- testing...
08 June 2018 2,194 4 View
