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Hi everyone, I made a mistake when I computed how much antibody I needed for my FACs, so I have an excess of exactly 10 reactions. Since they are so expensive and our lab is so poor, I was...
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Hello fellow researchers, I am having trouble with H&E staining. My slides end up blurry and unable to focus. I am following what I was taught, samples fixed in 4% PFA overnight, sucrose...
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I am developing a FACs B cell panel for my experiments and will need about 7-8 colours. We use C57BL/6 mice. I am trying to match my fluorochrome brightness and size to the abundance of markers on...
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Dear All, I am taking over the Master's student's project and I am a new PhD student. We have experiments involving tumour implantation in mice. The MSc student is used to scraping cells down...
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Dear Researchers, I need a comprehensive guideline to follow to determine analysis cut-off points (e.g.: number of nuclei per sample, percentages to consider a deletion present, false +/- testing...
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