Dante Johnson

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Questions related from Dante Johnson

Does anyone have a protocol for loading live mammalian cells (specifically HEK293) with Unlabeled Phalloidin and Cytochalasin B?

This is not for microscopy studies. This is for an in cell protein foot printing technique, Fast Photochemical Oxidation of Proteins FPOP. I just need guidance on how to get the unlabeled...

04 April 2018 429 2 View

Issues with resolubilizing Alph Synuclein Protein (Fibrils)

I want to digest my alpha synulein protein (monomers and fibrils). After disaggregation of the fibrils and drying via speedvac, I tried resolubilizing the fibril sample with 20ul 8M...

08 August 2017 8,250 4 View