Daniyal J Jafree

3 Questions 1 Answers 0 Followers

Questions related from Daniyal J Jafree

Safety issues concerning tetrahydrofuran for dehydration in 3D imaging protocols?

I am thinking of purchasing tetrahydrofuran (THF) for use in tissue dehydration of transgenic (tdTomato-expressing) mouse organs prior to tissue clearing using lightsheet fluorescence microscopy...

31 August 2020 8,644 1 View

Troubleshooting for vibratome cutting?

I’m currently having issues with cutting thick sections (~1mm) or mouse / human pathological samples on our Leica vibratome. I’ve described my procedure below. As opposed to getting clean cuts,...

29 January 2019 6,866 0 View

ScaleA2 clearing and confocal imaging - freezing samples / timing of immunostaining?

Hi! I am planning to do some whole mount immuno for mouse embryonic tissue. I read about ScaleA2 and it seems like a good yet cheap option, particularly as it will preserve fluorescent proteins...

06 October 2017 9,987 2 View