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Does anyone work with expression in S2 cells? How efficient is the transfection using Lipofectamine 2000? Everytime I try to transfect using CaCl2 I get a cloudy media, and it seems that the...
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Is it possible to perform a PCR, using as the reverse primer a oligo dT, the same used in the reverse trancription? I am trying to amplify a mRNA, but with no success
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Does anyone has a denaturing RNA gel protocol, by just adding guanidine to the sample, and running in a regular agarose gel?
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