Danielle Snider

3 Questions 2 Answers 0 Followers

Questions related from Danielle Snider

Does anyone have a tried and true in vitro Plasmodium falciparum culture synchronization method that they are willing to share?

I am trying to set up an experiment with a tightly synchronized P. falciparum culture to detect changes in expression levels of genes that are present during a particular stage of the parasite...

07 January 2019 7,983 3 View

For culturing P. falciparum gametocytes in vitro, what is considered a 'low passage number'?

I'm hoping someone with experience working with P. falciparum in vitro can help me with this! I'm starting in vitro work with P. falciparum, and focusing on culturing gametocytes. I'll be...

06 June 2018 314 2 View

How can I troubleshoot the issues I am encountering with P. falciparum RNA Extraction from Dried Blood Spots?

I was hoping to get some input regarding the process I use to extract P. falciparum mRNA from dried blood spots for use in qPCR. I am trying to amplify Pfs25 mRNA, and I've been running into two...

31 October 2017 8,757 2 View