Danielle Brotto

4 Questions 6 Answers 0 Followers

Questions related from Danielle Brotto

ChIP-seq using anti-HA antibody. Is it necessary to sequence the empty vector control?

Hello everyone, I am performing ChIP seq in tumor cell lines to find target genes of a specific transcription factor. There is no antibody for my protein, so I am using anti-HA antibody. For each...

17 February 2020 6,233 0 View

How to isolate syncytiotrophoblasts and cytotrophoblasts from placenta?

Hi! I need to isolate placenta cells in culture, syncytiotrophoblasts and cytotrophoblasts, specifically. Does anyone knows a protocol to do it with high grade of purity from term placentas?

03 February 2016 2,521 2 View

How to analyse the data of TaqMan copy number assay.

When I export the data from real-time to the software copycaller some plots are between 1 and 2 numbers of copies, how do I know if the sample contains 1 or 2 copies of my gene of interest?

12 April 2013 1,250 5 View

ChIP-seq in placental tissue – Optimization of sonication.

I need to do ChIP-seq for a transcription factor in human placenta. I have done it dozens of times, changing reagents and experimental conditions, but I have not yet achieved satisfactory...

01 January 1970 3,815 8 View