Danielle Baum

5 Questions 1 Answers 0 Followers

Questions related from Danielle Baum

Looking for consensus sequences using prosite or any other platform?

I am currently trying to look for a consensus sequence that's been identified to be a site of phosphorylation by a specific kinase. I am currently using ScanProsite to try and identify whether...

05 April 2021 3,420 1 View

Anyone with experience conducting proximity ligation assay (PLA) in mouse heart tissue ?

I was wondering if anyone had any experience doing proximity ligation assays (PLA) using mouse whole heart (ventricular) tissue (in any state)?

30 November 2020 9,268 1 View

IPSC-cardiomyocytes being paced for calcium transience assay are dying?

After infecting iPSC-cardiomyocytes with GCaMP6f for a calcium transience assay using a field stimulation pacer at 0.2 Hz, I notice the cells dying after about 6-12 hours. The auto microscope used...

27 January 2020 6,904 0 View

Mouse islet isolation without pancreas perfusion?

Is there a current/reliable way to isolate mouse islets (keeping their morphology and viability for culturing in vitro) without perfusing the pancreas through the bile duct?

15 September 2019 3,716 1 View

Amplify 72% GC rich with PCR?

Update: Thank you everyone--what I should have included was that the fragment forms tetra-G structures. It's a 600 bp long sequence with 80 repeat of GGGG (with 4 non-G base pairs in between)....

20 July 2018 8,642 7 View