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Questions related from Daniela Eletto
I am trying to grow an unstable plasmid (PERK, transmembrane protein of the endoplasmic reticulum) but the amount of DNA that I usually get from bacterial cultures is always too low (sometime...
09 September 2013 6,518 7 View
We would like to find an alternative to the Lipofectamine which is very efficient but quite expensive. Since in my lab we used to buy 2 tubes of LIPOFECTAMINE every week , we would like to cut...
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I am trying to extract RNA from cells (to do a RT-PCR) and usually it works, but now it stopped working and the RNA is always degraded. I use a Trizol-based extraction. So far, I changed every...
01 January 2013 744 28 View
I am trying to follow the phosphorylation profile of a protein (with one single phosphorylation site) under chemical treatment through phos-tag gel. I've been using two protocols: one based on...
01 January 2013 4,220 70 View
I successfully performed RT-PCR on different cell lines several times, but now it is not working anymore. Some cDNA are amplified while others are not. So, first I changed the enzymes (either for...
12 December 2012 7,335 30 View
I'm trying to pulldown a myc-tagged protein and I have been using myc-beads (already conjugated) but the IP was dirty (the Co-purified protein was also in the negative control). So then I tried a...
12 December 2012 3,819 41 View
