Daniela Ross-Kaschitza

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Questions related from Daniela Ross-Kaschitza

What is the best (or easiest) method to quantify apoptotic yeast cells?

As Caspase 3 assay is not applicable in S. cerevisiae, so what would be a good method to measure apoptosis/PCD in yeast cells? We would be interested to have a simple method (maybe simpler than...

09 September 2014 9,394 6 View

Protein "smear" in acidic region of 2D-GE Western Blot.

I am interested to see different isoforms of a protein on a 2D-Blot. The 2D-GE or Blotting itself work nicely (I can see nice patterning after Blotting using PonceauS), but my protein of interest...

14 June 2013 8,067 5 View

What is your experience with reference genes for RT-qPCR in Saccharomyces cerevisiae?

So far we are using Act1 to normalize qPCR data of yeasts with mutations in translation initiation factors.

05 June 2013 8,641 5 View