How to perform the embedding and cutting of 0,4 um transwell Pet inserts to visualize polarized cell layer for histology?

i seeded CACO2 on 0,4 um PET transwell insert, i want to see my cell layer in transversal view in order to monitor the polarization and formation of brush border. To do this, i fixed with 4% PFA...

17 April 2024 6,499 1 View