Daniel Weishäupl

3 Questions 23 Answers 0 Followers

Questions related from Daniel Weishäupl

Antibody recognizes peptide and purified protein but not overexpressed protein in lysate - How can I solve this?

Dear all, I generated an antibody against a quite hydrophobic peptide of a protein. The immunization worked and it is possible to detect the specific peptide in an ELISA. It is also possible to...

07 July 2016 8,760 5 View

Has anyone experience with antibodies against hydrophobic peptides?

We would like to create antibodies against a very hydrophobic region of a protein. The KLH linked peptide itself is absolutely insoluble and can only be solubilized by adding hydrophillic amino...

11 November 2014 3,827 13 View

Can anyone recommend a transfection reagent or method for transfection of MEF cells?

I am trying to transfect MEF cells with high transfection efficiency. I tried Lipofectamine 2000 and Attractene which both had a bad efficiency.

04 April 2013 3,754 14 View