Daniel Vik

3 Questions 12 Answers 0 Followers

Questions related from Daniel Vik

Are there alternatives to dissolve agar by boiling?

I am wondering if it is possible to dissolve agar by different means than boiling? I am planning an experiment where I want to move Arabidopsis seedlings grown on ½MS + chemical inducer to...

04 April 2016 2,396 5 View

Co-IP elution as a smear?

I am trying to do Co-IP using Pierce Crosslink Immunoprecipitation Kit. However, something is puzzling me. When I do the elution and subsequently visualize by western blot, I quite often get...

04 April 2013 3,752 11 View

How do you avoid denaturing antibodies when doing IP under denaturing conditions?

I am currently trying to precipitate a protein for which I have an antibody targeting a peptide epitope. However, so far it seems likely that the epitope is buried. I have heard that it is...

02 February 2013 6,659 4 View