8 Questions 41 Answers 0 Followers
Questions related from Daniel Nii Aryee Tagoe
I am working on putative proteins. I need free online software and sites to determine the structures. Any suggestions.
07 July 2013 2,579 3 View
After transfection, do you select before the first division of your cells or after? If you have tried both, which do you think worked best?
06 June 2013 8,825 6 View
I have GFP tagged protein under expression in a cell. However, the whole cell seems to light up with the GFP although l expect the protein to localized at only certain areas. I used a normal...
06 June 2013 9,762 35 View
Has anyone had a problem with an Over Expression (O.E) line with either GFP, N or C terminal His or Myc tags. Did they affect the phenotype and if so, to what extent?
05 May 2013 9,245 7 View
In a Tet inducible over expression assay how much Tetracycline is enough? Does one need to just follow the literature?
05 May 2013 2,851 27 View
I am presently using the gateway cloning system. Having managed to generate my own gateway compatible vector, l want to make my final construct. BP rxn was perfect. However, LR rxn is not working....
05 May 2013 5,872 14 View
At a recent conference, there was a debate on whether young scientists who leave their countries to study should be compelled to return. One scientist insist that since scientific discoveries are...
04 April 2013 8,435 5 View
l am presently trying to ligate a gene (2.7kb) into a 6.2kb vector. The problem is my my gene have restriction sites which are the same as the one in the vector. Complimentary enzymes does not...
04 April 2013 7,026 20 View
