Daniel Pp

3 Questions 3 Answers 0 Followers

Questions related from Daniel Pp

How do I design primers without exact the DNA sequence?

So, I am trying to do gene-knockout in Burkholderia ubonensis by using Splicing by overhang extension (SOE) PCR, and I am having problems with designing working primers. This is because our lab...

08 August 2017 9,563 2 View

Is it possible to do transformation without using plasmid?

Is it possible to do transformation without using plasmid? Can I directly force DNA fragments from PCR into a bacterium without ligating it into a plasmid? And how do I do it? (I am not using E.coli)

18 July 2017 7,157 8 View

Is it possible to do transformation without using plasmid?

Is it possible to do transformation without using plasmid? Can I directly force DNA fragments from PCR into a bacterium without ligating it into a plasmid? And how do I do it? (I am not using E.coli)

07 July 2017 5,667 10 View