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The final mRNA products in Eukaryotes are always short than the DNA sequence it was transcribed from due to introns splicing. If we take an eukaryote's gene, put in bacteria and expresses it, then...
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So, I am trying to do gene-knockout in Burkholderia ubonensis by using Splicing by overhang extension (SOE) PCR, and I am having problems with designing working primers. This is because our lab...
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Is it possible to do transformation without using plasmid? Can I directly force DNA fragments from PCR into a bacterium without ligating it into a plasmid? And how do I do it? (I am not using E.coli)
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