Daniel Osorio

3 Questions 18 Answers 0 Followers

Questions related from Daniel Osorio

Immunoprecipitation of ZZ tag protein, can someone offer advice on reagents?

I need to do a pull-down/ IP with a purified zz tagged protein. In a reference paper they used IgG sepharose (brand not mentioned). I would like to go for magnetic beads, I saw you can use...

09 September 2013 3,745 4 View

Appropriate Control vectors for Gateway DEST plasmids?

I would like to know what you use for expression controls of destination gateway vectors for example in IP or transfection. Let me illustrate with an example, for example you want to express...

04 April 2013 8,126 4 View

Is it possible to express and purify non-muscle myosin II S1 fragment from bacteria?

It does not seem to be very common, either it is extracted from natural sources and cleaved or expressed using Dityostelium or baculovirus

01 January 1970 4,778 3 View