Daniel Chaves de Lima

5 Questions 10 Answers 0 Followers

Questions related from Daniel Chaves de Lima

Why I cannot visualize my over-expressed surfactant protein on SDS-PAGE neither purify it?

Hello, folks. I am working in the characterization of a putative biosurfactant protein from Archaea. I screened out the gene from a metagenomics library, cloned it in expression vector with a...

05 May 2017 5,406 3 View

Does E. coli K-12 derived mutants express genes driven by lac promoter?

Hello. I am working with a sodC mutant (JW1638) and according to Coli Genetic Stock Center, it's derived from K-12. Here is the genotype: F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-,...

08 August 2015 4,382 3 View

How can I infer that two genes are paralogous using phylogenetic analysis?

Hello, everyone. I have two genes that are known to be paralogues (in bacteria) although I've never seen any work in the literature proving this assumption. I would like to conduct a phylogenetic...

05 May 2015 5,153 7 View

Which plasmids should I use in a Complementation test?

Hello. I have a sodC (E. coli) mutant and I want to verify if a specific gene complements the sodC deletion. Which kind of plasmid should I use to clone this gene? It can be a plasmid with...

11 November 2014 4,296 3 View

How do I determine Molar Concentration of Nucleic Acids in number of nucleotides and in numbers of molecules?

I've been looking for a way to understand how to determine the molarity of nucleic acids in numbers of nucleotides and in numbers of molecules. For example: if I get the size, in base pairs, of...

05 May 2014 875 7 View