Daniel E Gracilla

6 Questions 8 Answers 0 Followers

Questions related from Daniel E Gracilla

Is it possible that cell line (OSCC) will grow symbiotically with bacteria?

I observed some moving particles "'not sure if bacteria" in some OSCC cells in culture,. The cells are growing well despite on those moving materials.

03 March 2018 5,441 4 View

Any idea how to troubleshoot my problem with target stable cell lines.?

Good day!I am currently generating stable cell lines( KON ) using TTA vector and G418 as drug selection agent.?After two weeks i got isolated colony from some wells at the 900ug/ml (G418). I...

03 March 2018 5,004 5 View

What is the best strategy to knock in cell line using cas9 system?

Is homology sequence recombination will work? Any construct suggestion. Thanks in advance..

03 March 2018 1,957 1 View

Good day! I am doing drug screening for Oral squamous Carcinoma cell Lines. My drug G418 concentration is from 100 ug to 100ug per ml?

It turns out that my cells become 100 percent (from 70% confluent) confluent the after 4 days? Any suggestion On optimizing drug screening.?

02 February 2018 4,805 2 View

Any idea regarding mutagenesis. Overlap extension PCR?

I am trying to amply a 1400bp gene.. My expected fragments which are 900 and 500 were achieved. I joined the two fragments and i got 1400 as expected. This is my mutation desire CAG to GAG....

01 January 2018 3,095 6 View

Good day. Anyone can help me regarding how much volume on the fragments should I use for overlap extension PCR?

The two fragments have the same intensity. Any detailed PCR Reaction for this. Thank you.

12 December 2017 6,973 3 View