Daniel Brady

2 Questions 5 Answers 0 Followers

Questions related from Daniel Brady

What resuspension buffer to use for sgRNA and Cas9 nuclease?

Hello, I'm preparing to preform CRISPR/Cas KO by microinjection in the silkworm Bombyx mori. I'm ordering synthetic sgRNA and using SpCas9 nuclease for this experiment. I've read that when...

01 January 2019 8,068 0 View

How can I estimate cDNA quantity produced by RT-PCR?

Hello, I'm trying to find an equation for estimating the quantity of cDNA produced by RT-PCR. However, I keep getting funnelled into qPCR threads and forums, that I'm not finding helpful. I'll...

10 October 2017 7,250 5 View