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We are working with ipsc-derived cardiomyocytes and according to some protocols they prefer hypoxic conditions rather than normoxic. Our incubators can maintain only CO2 concentrations. So is...
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When I pick colonies from Geltrex and transferring to Geltrex matrix, they don't attach to plate. Should colonies be well washed from Geltrex ? Or may be dispase or collagenase should be used?
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Our packaging cell line was infected with mycoplasma, and our virus-containing media also contains mycoplasma. It is recommended to discard it, but is there any other way? We've thought to...
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