What are some possible technical failures during lentiviral packaging using HEK293T?

I'm having trouble packaging lentivirus in HEK293T using pCMV, pMDG, an shRNA and polyfect. I normally seed 1.5 x 106 HEK293T cells in 60 mm on Day 0, which translates to about 60 to 70%...

21 May 2024 9,046 1 View